DNA-based biosensors are powerful tools for detecting specific nucleic acid sequences and other biomolecules. However, challenges such as probe degradation, nonspecific binding, and steric hindrance often limit their sensitivity and reliability, especially in complex biological fluids.
This thesis will explore the use of PEGylated DNA probes—DNA strands modified with polyethylene glycol (PEG) chains—to overcome these challenges. PEGylation enhances probe stability, reduces nonspecific adsorption, and improves hybridization efficiency by increasing probe accessibility.
The work will include:
Functionalization of sensor surfaces with PEGylated DNA probes
Characterization of probe immobilization, stability, and hybridization efficiency
Evaluation of biosensor performance in buffer and complex biological fluids
Optimization of PEG linker length and density to maximize sensitivity and specificity
This project aims to advance the development of robust and sensitive biosensors suitable for real-world biomedical applications.